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SHARED INSTRUMENTS
Training on these common-use Instruments is provided
in several forms, including arranging formal presentations by technical
representatives or small group trainings conducted by the facility
personnel. These instruments are mostly located in building two (rm 2200).
The FUJI
FLA-5100 imaging system allows imaging of fluorescent
and radioisotopic samples as large as 40 x 46cm at pixel size as low as
10 microns. 
The
Roche LightCycler utilizes a rapid air heating and cooling
system to decrease run times to approximately 30-45 minutes. With a 32
sample capacity, this instrument is perfect for small projects or as a
quick diagnostic tool. This instrument is located in (Building 7 room 7102)
The
Stratagene Mx3000p is based on
a peltier 96-well block system and an overhead scanning optics system.
Although a typical 40-cycle run on this instrument will take 2-2.5
hours, capacity is 96 samples, allowing for a greater number of
replicates and internal controls. This
system is recommended for those researchers that intend on completing a
relatively large experiment or a project that requires the processing of
replicates and standard curves for more accurate analysis.
Turner
Veritas 96 well Luminometer is a state-of-the-art microplate
luminometer with high sensitivity and broad dynamic range for all
chemiluminescent and bioluminescent applications. Equipped with two
variable volume injectors, the Veritas is capable of performing both
flash and glow type luminescent assays. It
is approximately 5-fold more sensitive than the previous common-use
Berthold luminometer. The software is easy
to use, and is pre-programmed for all Promega assays.
Data are automatically exported to Excel.
The
Alpha imager from Alpha Innotech (various locations) allows
documentation of gels or other objects with UV or visible light. Data can be saved as thermal prints or
electronic files (TIFF). Software features
include quantitation, annotation, and even colony counting. Excellent short videos covering many
aspects of Alphaimager use, including image acquisition, enhancement,
annotation, and quantitation, are at Lee Hedges web site .
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