Electrophysiology Core in Burnham
Institute
The Electrophysiology Core in
Burnham Institute is a part of Neuroscience Center Core facility. Together with its sibling facility in Salk
Institute, run by Dr. Stephen F. Heinemann, the core will provide services for
projects using electrophysiological approaches.
Administration and Operation
Dr. Zhang will oversee the
Core facilities and ensure that the equipment is used appropriately. All
projects will be subjected to approval and accommodated in the order they
arise. A staff scientist will conduct experiments for accepted projects and for
training.
Equipment
The Electrophysiology Core provides access
to a slice and a patch-clamp rig for electrophysiological recordings from brain
slices and cell cultures, respectively. In addition, the Core is equipped with
an oocyte recording rig.
Services
To compliment our sibling
facility in Salk institute, we will mainly provide services for experiments
using cell cultures and oocytes. These
systems are best for studying drug/reagent interaction with receptors, native
or recombinant, expressed in cell surfaces. The Core will assist each project
with the experimental design, data collection and data interpretation. We will
also train qualified personnel for conducting experiments themselves. We will
accommodate projects using slice recordings if our sibling facility is not
available at the time. Examples of services are listed below:
1).Patch-clamp recording from single cells include primary neuronal
cultures or cell lines
2).Voltage-clamp
recording from oocytes expressing recombinant receptors
3).Extracellular or
patch-clamp recording from Brain Slices
Service charges
1)
Project performed by core personnel: $60/hour.
2)
Training fee: $100/hour.
3)
Project performed by non-core personnel: $30/hour.
Contact information
Dongxian Zhang, Ph.D.
Burnham Institute for Medical
Research
Building7, Rm 7203
10901N. Torrey
E-mail: dzhang@burnham.org
Phone: 858-795-5263